MAK518

ADP Assay Kit

sufficient for 100 fluorometric tests

• Synonym(s): ADP Detection Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• detection method: fluorometric
• relevant disease(s): hematological disorder
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.

Features and Benefits

• As low as 0.1 µM ADP can be quantified.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for ADP determination in cells and other biological samples.

Principle

The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H290,H314,H335,H411
• pcodes: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 8A - Combustible corrosive hazardous materials
• wgk_germany: WGK 2