P3889
Polygalacturonic acid
≥85% (titration), from oranges
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About This Item
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biological source
oranges
Quality Level
assay
≥85% (titration)
form
powder
color
white to brown
mp
60 °C
solubility
aqueous NaOH: 1%, clear to hazy
storage temp.
room temp
SMILES string
O1[C@@H]([C@@H]([C@H]([C@H]([C@H]1C(=O)O)O)O)O)O
InChI
1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1
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General description
Application
- in the preabsorption of 2F4 primary antibody solution[3]
- as a substrate to determine polygalacturonase activity[4]
- in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases[5]
Biochem/physiol Actions
Other Notes
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
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