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P3889

Sigma-Aldrich

Polygalacturonic acid

≥85% (titration), from oranges

Linear Formula:
(C6H8O6)n
CAS Number:
MDL number:
NACRES:
NA.25

biological source

oranges

Quality Level

Assay

≥85% (titration)

form

powder

color

white to brown

mp

60 °C

solubility

aqueous NaOH: 1%, clear to hazy

storage temp.

room temp

InChI

1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1

InChI key

AEMOLEFTQBMNLQ-BKBMJHBISA-N

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This Item
T740781325PHL83896
Polygalacturonic acid ≥85% (titration), from oranges

Sigma-Aldrich

P3889

Polygalacturonic acid

Trigalacturonic acid ≥90% (HPLC)

Sigma-Aldrich

T7407

Trigalacturonic acid

Polygalacturonic acid ≥90% (enzymatic)

Sigma-Aldrich

81325

Polygalacturonic acid

Polygalic acid phyproof® Reference Substance

PHL83896

Polygalic acid

assay

≥85% (titration)

assay

≥90% (HPLC)

assay

≥90% (enzymatic)

assay

≥95.0% (HPLC)

form

powder

form

powder

form

powder

form

-

mp

60 °C

mp

-

mp

-

mp

-

solubility

aqueous NaOH: 1%, clear to hazy

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

General description

Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.

Application

Polygalacturonic acid has been used:
  • in the preabsorption of 2F4 primary antibody solution
  • as a substrate to determine polygalacturonase activity
  • in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases

Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca2+ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.

Biochem/physiol Actions

In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Zhangyong Dong et al.
BMC biochemistry, 12, 51-51 (2011-09-17)
Fusarium wilt is an economically devastating disease that affects banana production. Although Cavendish banana cultivars are resistant to Fusarium oxysporum f.sp. cubense race 1 (FOC1) and maitain banana production after Gros Michel was destructed by race 1, a new race
Bacterial Wilt Disease: Molecular and Ecological Aspects, 171-171 (1998)
Marianne Hiorth et al.
International journal of pharmaceutics, 308(1-2), 25-32 (2005-12-06)
This study has investigated the potential of immersion coating calcium containing pellet cores first with pectin, and then with two different cross-linkers, calcium or chitosan. The interaction between pectin and calcium, and between pectin and chitosan, are believed to slow
Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan
Bonnin E, et al.
Carbohydrate Polymers, 74(3), 411-418 (2008)
Kun Cheng et al.
Drug development and industrial pharmacy, 30(4), 359-367 (2004-05-11)
Insulin-loaded calcium pectinate nanoparticles were prepared as a potential colonic delivery system by ionotropic gelation with calcium ions. The effects of pectin molecular weight (Mv) and formulation pH on the characteristics of the nanoparticles were evaluated. Commercial pectins, LM101 and

Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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