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P7002

Sigma-Aldrich

Phospho(enol)pyruvic acid trisodium salt hydrate

≥97% (enzymatic), powder

Synonym(s):

2-(Phosphonooxy)-2-propenoic acid trisodium salt, PEP

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About This Item

Linear Formula:
C3H2O6PNa3 · x H2O
CAS Number:
5541-93-5
Molecular Weight:
233.99 (anhydrous basis)
MDL number:
MFCD00150734
UNSPSC Code:
12352204
PubChem Substance ID:
24898810
NACRES:
NA.32

product name

Phospho(enol)pyruvic acid trisodium salt hydrate, ≥97% (enzymatic)

Quality Level

200

assay

≥97% (enzymatic)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

[Na].OC(=O)C(=C)OP(O)(O)=O

InChI

1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;

InChI key

KZPUEZVJSLLABH-UHFFFAOYSA-N

Application

Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:
  • several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
  • in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
  • as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions

Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.
Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kento Tokuyama et al.
Biotechnology and bioengineering, 115(6), 1542-1551 (2018-02-20)
Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of
Casimir Ledoux Sofeu et al.
PLoS neglected tropical diseases, 12(9), e0006597-e0006597 (2018-09-07)
Canine rabies is endemic in Cameroon, but human rabies exposures and cases are likely underreported because of inadequate surveillance. In 2014, the surveillance network in the West region of Cameroon was reinforced by introducing a new anti-rabies center, a framework
Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons
Azad R, et al.
ScienceOpen Research (2015)
Timothy R Rudd et al.
Faraday discussions, 218(0), 303-316 (2019-05-28)
A biological medicine (or biologicals) is a term for a medicinal compound that is derived from a living organism. By their very nature, they are complex and often heterogeneous in structure, composition and biological activity. Some of the oldest pharmaceutical
Yousuke Nakai et al.
Gastrointestinal endoscopy, 81(1), 119-126 (2014-12-03)
Wire-guided cannulation (WGC) was reported to decrease post-ERCP pancreatitis (PEP), but risk factors for PEP in WGC are not fully elucidated. To evaluate the incidence and risk factors of PEP in WGC. Single-center retrospective study. Academic center. A total of
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