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PCRISPR005

Sigma-Aldrich

Human CRISPR Inhibition 10x Compatible Druggable Library

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packaging

pkg of 5 vials (5x200µL aliquots )

concentration

≥5x108 VP/ml (via p24 Assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

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PCRISPR007PCRISPR008CRISPRILIB
concentration

≥5x108 VP/ml (via p24 Assay)

concentration

≥5x108 VP/ml (via p24 Assay)

concentration

≥5x108 VP/ml (via p24 Assay)

concentration

-

application(s)

CRISPR

application(s)

CRISPR

application(s)

CRISPR

application(s)

CRISPR

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

The 10x Compatible Human CRISPRi Kinase Phosphatase Drug Targets Kit (Druggable Genome) contains one sub-pool of the top two ranked sgRNAs per gene for increased sensitivity and includes non-targeting controls built-in. In addition, the LV15 vector has both BFP and puromycin as selection markers and an optimized scaffold with capture sequence 2 (CS2, Compatible with 10x Feature Barcode technology) for sgRNA expression and function.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Application

  • Functional Genomics/Target Validation
  • Focused forward genetic activation screening
  • Validated positive and negative controls
  • Set up and optimization of screen assay
  • Creation of cell lines stably expressing KRAB-dCas9

Features and Benefits

  • Focus on your research, and we will generate your lentivirus screening library.
  • Optimized (F+E) gRNA scaffold. (pools are gRNA-only, KRAB-dCas9 sold separately CRISPRICON or CRISPRI10X
  • A total of 2,318 Human Druggable genes targeted.
  • Total of 4,636 unique gRNAs (top two ranking gRNAs)
  • Use puromycin and or BFP as selection markers

Principle

The power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease. CRISPR is most commonly used to create double-stranded breaks that often result in loss of gene function (CRISPR-KO). However, the full extent of CRISPR′s utility extends beyond just targeted cutting of DNA. CRISPR′s Nuclease-independent applications provide equal targeting specificity. Instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown complements CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi. As a world leader in producing lentiviral libraries, we design and build custom pools to any specifications. With this capability, we have partnered with 10x Genomics to bring innovative pooled screening products to enable discovery at single-cell resolution

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Max A Horlbeck et al.
eLife, 5 (2016-11-04)
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predict highly effective single guide

Articles

CRISPR lentiviral screening libraries are powerful research tools. Continuing a decade long commitment to library screening manufacturing, we have partnered with 10x Genomics to bring 10x Feature Barcode-compatible pooled screening products to you.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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