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PP0500

Sigma-Aldrich

Protease Profiler Kit

Proteases for Mass Spectrometry and Proteomics

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NACRES:
NA.56

quality

Proteases for Mass Spectrometry and Proteomics

Quality Level

storage temp.

−20°C

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PP0100P8465539125
Protease Profiler™ Kit Proteases for Mass Spectrometry and Proteomics

PP0500

Protease Profiler Kit

Trypsin Profile IGD Kit

PP0100

Trypsin Profile IGD Kit

Sigma-Aldrich

P8465

Protease Inhibitor Cocktail powder

Sigma-Aldrich

539125

Protease Assay Kit

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

For detailed characterization of proteins of interest, increase your protease selection with the Protease Profiler. The Protease Profiler provides four proven alternative proteases in addition to Proteomics Grade Trypsin, an enzyme solublization reagent, and an enzyme reaction buffer. Each component has been stringently purified and qualified for MALDI-MS to ensure optimal performance. Characterize with greater flexibility, perform double enzymatic digestions, and explore alternative cleavage sites with the Protease Profiler.
  • Double Enzymatic Digestion - An initial digestion may result in peptide fragments greater than 5 kDa, often above the optimum detection range for most MS systems/instruments. A second digestion using a protease with different specificity results in cleavage of the large peptides, generating smaller peptides suitable for MS.
  • Explore Alternative Proteases - Trypsin may be incompatible with some protein samples. Find the protease best suited to your protein of interest and improve the quality of your data.
  • Perform Either Solution or Gel-based Digests - Optimized protocols and reagents for both digest conditions are provided, enabling flexibility in your analysis.

Application

The Protease Profiler Kit can be used for different enzymatic digestions on the proteins of interest.

Storage and Stability

The entire Protease Profiler Kit retains activity for at least 1 year when stored at –20 °C. Alternatively, some of the components (Trypsin, Lys-C, Asp-N, Trypsin Solubilization Reagent, and Enzyme Reaction Buffer) may be stored at 2–8 °C. The storage temperature indicated on the labels of these products is 2–8 °C. Storage at –20 °C will not compromise the performance of these products.

Legal Information

Protease Profiler is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Enzyme Solublization Reagent 1 mL

  • Enzyme Reaction Buffer 25 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS

  • P3303Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder 2 μgSDS

  • P6181Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder 25 μgSDS

  • P6056Endoproteinase Arg-C from mouse submaxillary gland, suitable for protein sequencing, lyophilized powder 5 μgSDS

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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John H Beattie et al.
Proteomics, 8(10), 2126-2135 (2008-05-21)
Human zinc deficiency is a global problem and may influence the development of cardiovascular disease. Our objective was to determine Zn deficiency affected pathways and protein interactions in rat aorta and their likely influence on stress-induced atherogenesis. In two separate
Maria C Romero-Puertas et al.
The Plant cell, 19(12), 4120-4130 (2008-01-01)
Nitric oxide (NO) is a free radical product of cell metabolism that plays diverse and important roles in the regulation of cellular function. S-Nitrosylation is emerging as a specific and fundamental posttranslational protein modification for the transduction of NO bioactivity

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