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R7904

Sigma-Aldrich

Anti-Rab5 antibody, Mouse monoclonal

clone Rab5-65, purified from hybridoma cell culture

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Synonym(s):
Anti-RAS-associated Protein RAB5A
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Rab5-65, monoclonal

form

buffered aqueous solution

mol wt

~24 kDa

species reactivity

human, cat, bovine, mouse, rat

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK
indirect ELISA: suitable
microarray: suitable
western blot: suitable

isotype

IgG3

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

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Anti-Rab9 from rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

clone

Rab5-65, monoclonal

clone

polyclonal

clone

Rab7-117, monoclonal

clone

polyclonal

species reactivity

human, cat, bovine, mouse, rat

species reactivity

human, mouse, rat

species reactivity

bovine, human, monkey, mouse, chicken, rat

species reactivity

rat, mouse, human

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

Gene Information

human ... RAB7A(7879)
mouse ... Rab7(19349)
rat ... Rab7a(29448)

Gene Information

human ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)

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General description

Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.
Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.

Immunogen

synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.

Application

Anti-Rab5 antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • co-immunoprecipitation assay
  • western blotting
  • immunofluorescence

Biochem/physiol Actions

Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).
Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Probing the druggability of membrane-bound Rab5 by molecular dynamics simulations
Edler E and Stein M
Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 434-443 (2017)
Pamela A Godoy et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 142, 111968-111968 (2021-08-04)
Amyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer's Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic
The Autophagic Process Occurs in Human Bone Metastasis and Implicates Molecular Mechanisms Differently Affected by Rab5a in the Early and Late Stages
Maroni P, et al.
International Journal of Molecular Sciences, 17(4) (2016)
Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7
Zhao P, et al.
Biochimica et biophysica acta. General subjects, 1863(2), 502-510 (2019)
Increased expression of Rab5A predicts metastasis and poor prognosis in colorectal cancer patients
Yu MH, et al.
International Journal of Clinical and Experimental Pathology, 8(6), 6974-6980 (2015)

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