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RAB0219

Sigma-Aldrich

Human sICAM1 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.313-20 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ICAM1(3383)

General description

The Human sICAM-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human soluble ICAM-1 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human ICAM1

Application

For research use only. Not for use in diagnostic procedures.
Human sICAM1 (intercellular adhesion molecule 1) ELISA Kit has been used for measuring sICAM1 levels in the endothelial cell-conditioned medium.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

The expression of ICAM1 (intercellular adhesion molecule 1) is strongly increased by Interleukin-1 (IL-1) and interferon (IFN)-γ. It is expressed on lymphoid cells where it is required for phorbol ester-induced aggregation. It participates in LFA-1-dependent NK cell stimulation through its attachment to actin cytoskeleton through α-actinin or ezrin. It also acts as the receptor for rhinovirus, a common cold virus, and Plasmodium falciparum. Soluble ICAM-1 (sICAM1) levels are elevated in pregnant women with gestational diabetes mellitus (GDM), which is an outcome of endothelial cell activation and dysfunction. In addition, ICAM-1 also has an essential role in binding circulating leukocytes to the blood vessel wall and trans-endothelial migration to vascular intima. Meta-analysis of case-control studies show that rs1799969 polymorphism in ICAM-1 gene is linked with increased susceptibility to cancer. sICAM1 might act as a biomarker for unipolar and bipolar patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Theresa Chikopela et al.
Southern African journal of HIV medicine, 22(1), 1298-1298 (2021-12-04)
Cardiovascular disease (CVD) prevalence is rising among persons with HIV (PLWH) in sub-Saharan Africa. Oxidative stress and endothelial activation, resulting in reduced vascular compliance, are contributors to CVD risk. However, there is a paucity of vascular health data in this
Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway.
Chen ML
Autophagy, 9(12), 2033-2045 (2013)
Soluble Intracellular Adhesion Molecule-1 in Patients with Unipolar or Bipolar Affective Disorders: Results from a Pilot Trial.
Schaefer M
Neuropsychobiology, 74(1), 8-14 (2016)
The ICAM-1 Gly241Arg Polymorphism is Not Associated With Polycystic Ovary Syndrome - Results from a Case Control study in Kashmir, India.
Yousuf SD
Asian Pacific Journal of Cancer Prevention, 17(3), 1583-1588 (2016)
Intercellular Adhesion Molecule and Endogenous NOS Inhibitor: Asymmetric Dimethylarginine in Pregnant Women with Gestational Diabetes Mellitus.
Poniedzialek-Czajkowska E
Journal of Diabetes Research, 2016:1342643 (2016)

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