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GenElute Plasma/Serum RNA Purification Maxi Kit

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NA.52

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ambient

storage temp.

room temp

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room temp

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15-25°C

General description

Sigma′s GenElute Plasma/Serum RNA Purification Maxi Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 2 mL and up to 5 mL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Sigma′s GenElute Plasma/Serum RNA Purification Maxi Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual. Normally, the RNA yield from plasma or serum RNA is highly variable (ranging from 1 to 100 ng/mL).

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma/serum input ranging from 2 mL to 5 mL
  • No phenol extractions
  • No carrier RNA
  • Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL
  • Purify high-quality RNA in 35-40 minutes

Application

  • Reverse transcription qPCR
  • Reverse transcription PCR
  • Northern blotting
  • RNase protection and primer extension
  • Expression array assays
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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