S0698

Anti-SmB antibody, Mouse Monoclonal

clone 12F5, purified from hybridoma cell culture

• Synonym(s): Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 12F5, monoclonal
• mol wt: antigen ~25 kDa
• species reactivity: human, mouse, canine
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
• isotype: IgG1
• UniProt accession no.: P14678
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SNRPB(6628) ; mouse ... Snrpb(20638)

Description

General description

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.

SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

Immunogen

SmB recombinant protein.

Application

• Monoclonal Anti-SmB antibody produced in mouse has been used in:
• western blotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)