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S3504

Sigma-Aldrich

Sigmacell Cellulose

Type 20, 20 μm

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Synonym(s):
Cellulose, Cellulose powder, Cotton linters
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.56

type

Type 20

Quality Level

particle size

20 μm

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

Inchi Key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

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Application

Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.
High purity cellulose powders for partition chromatography.

Reconstitution

For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.

Legal Information

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stuart M Linton et al.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also
L Brinchi et al.
Carbohydrate polymers, 94(1), 154-169 (2013-04-03)
The use of renewables materials for industrial applications is becoming impellent due to the increasing demand of alternatives to scarce and unrenewable petroleum supplies. In this regard, nanocrystalline cellulose, NCC, derived from cellulose, the most abundant biopolymer, is one of
Yulia Fridman et al.
Genes & development, 28(8), 912-920 (2014-04-17)
Coherent plant growth requires spatial integration of hormonal pathways and cell wall remodeling activities. However, the mechanisms governing sensitivity to hormones and how cell wall structure integrates with hormonal effects are poorly understood. We found that coordination between two types
Okako Omadjela et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(44), 17856-17861 (2013-10-16)
Cellulose is a linear extracellular polysaccharide. It is synthesized by membrane-embedded glycosyltransferases that processively polymerize UDP-activated glucose. Polymer synthesis is coupled to membrane translocation through a channel formed by the cellulose synthase. Although eukaryotic cellulose synthases function in macromolecular complexes
Inka Vanwonterghem et al.
The ISME journal, 8(10), 2015-2028 (2014-04-18)
A replicate long-term experiment was conducted using anaerobic digestion (AD) as a model process to determine the relative role of niche and neutral theory on microbial community assembly, and to link community dynamics to system performance. AD is performed by

Protocols

To standardize an enzymatic assay procedure of cellulase.

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