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S5196

Sigma-Aldrich

Anti-Sirt1 antibody, Mouse monoclonal

clone SIR11, purified from hybridoma cell culture

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Synonym(s):
Anti-SIRTUIN1, Anti-Sir2α, Anti-mouse homologue of yeast Sir2
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

SIR11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~105 kDa

species reactivity

mouse

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of C-2 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Sirt1(93759)

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This Item
S5322S5447S5313
biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

mouse ... Sirt1(93759)

Gene Information

human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)

Gene Information

human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)

Gene Information

mouse ... Sirt1(93759)

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

SIR11, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Monoclonal Anti-Sirt1 (Sir2) (mouse IgG1 isotype) is derived from the hybridoma SIR11 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids at the C-terminus of mouse Sir2 with C-terminal added lysine. Sirt proteins are a family of proteins that are found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Sirtuin 1 (SIRT1) protein, a mammalian homolog of yeast Sir2, is a NAD-dependent histone deacetylase.

Specificity

Monoclonal Anti-Sirt1 (Sir2α) antibody is specific for Sir2α in mice.

Application

Anti-Sirt1 antibody, Mouse monoclonal has been used in:
  • western blot analysis
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemical methods

Monoclonal Anti-Sirt1 antibody is suitable for use in western blot (1-2 μg/mL using total cell extract of C-2 cells), immunoblot (approx. 105 kDa), microarray and indirect ELISA.

Biochem/physiol Actions

SIRT1 is a highly conserved sirtuin family deactelyase that modulates heart metabolism . SIRT1 has also been implicated in senescence and aging . Thus, SIRT1 may be used as a target for treating age-related diseases.
Sirt1 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes O-acetyl-ADP-ribose. Through histone deacetylation, Sirt1 may silence chromatin. It appears that Sirt1 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Sirt1 improves healthy ageing and protects from metabolic syndrome-associated cancer
Herranz D, et al.
Nature Communications, 1(28), 3-3 (2010)
Hepatic NAD+ deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing
Zhou CC, et al.
British Journal of Pharmacology, 173(15), 2352- 2368 (2016)
K Svensson et al.
Acta physiologica (Oxford, England), 221(3), 193-203 (2017-05-26)
Activation of the NAD+ dependent protein deacetylase SIRT1 has been proposed as a therapeutic strategy to treat mitochondrial dysfunction and insulin resistance in skeletal muscle. However, lifelong overexpression of SIRT1 in skeletal muscle does not improve parameters of mitochondrial function
Yu Wang et al.
Pharmacology & therapeutics, 136(3), 305-318 (2012-09-04)
Sirtuin 1 (SIRT1), the mammalian ortholog of yeast Sir2p, is a highly conserved NAD(+)-dependent protein deacetylase that has emerged as a key cardiometabolic regulator. During the past decade, Sir2p has been the focus of intense investigations and discussion because it
SIRT1 gene polymorphisms associated with decreased risk of atherosclerotic coronary artery disease
Nasiri M, et al.
Gene (2018)

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