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S8305

Sigma-Aldrich

Anti-Substance P Receptor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-NK1R, Anti-SPR

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa

species reactivity

human, rat, mouse, guinea pig

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (frozen sections): 1:5,000 using 4% paraformaldehyde (with picric acid/glutaraldehyde)-fixed, frozen, rat brain sections.
microarray: suitable
western blot (chemiluminescent): 1:2,000 using a rat brain membrane fraction extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TACR1(6869)
mouse ... Tacr1(21336)
rat ... Tacr1(24807)

General description

Substance P receptor (SPR), also known as neurokinin receptor 1 (NK-1 R), is a member of the G-protein-coupled class of seven transmembrane domain receptors. SPR gene with five exons, spanning 45-60kb on genomic DNA, is mapped to human chromosome 2. SPR is highly expressed in central nervous system.

Immunogen

synthetic peptide corresponding to the C-terminal of NK1R of rat origin (amino acids 393-407). This sequence is highly conserved in mouse, guinea pig, and human NK1R, but diverges in other tachykinin receptor subtypes NK2R and NK3R.

Application

Anti-substance P receptor antibody produced in rabbit has been used in immunohistochemistry.

Biochem/physiol Actions

Substance P (SP) exhibits pro-inflammatory, endocrine, neuromodulatory and trophic activities. Substance P receptor (SPR) upon binding to its ligand SP, facilitates extravasation of granulocytes and in inflammation and tissue derangement. In addition, activation of this receptor also enhances metabolism of phosphoinositol, leading to synthesis of inositol-1,4,5-trisphosphate and diacylglycerol which results in release of Ca2+ from the endoplasmic reticulum and activation of protein kinase C. SPR along with its ligand stimulates tumoral angiogenesis and cell proliferation in a variety of cancer types. Inhibition of substance P receptor retards the growth of neuroblastoma (NB) cell lines. Thus, SPR might act as a potential therapeutic target for a large variety of patients with neuroblastoma. SP-SPR complex is also implicated in the regulation of the pain transmission.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression of the NK-1 receptor on islet cells and invading immune cells in the non-obese diabetic mouse.
Persson-Sjogren S, et al.
Journal of Autoimmunity, 24, 269-279 (2005)
Abeer W Saeed et al.
The Journal of comparative neurology, 521(8), 1915-1928 (2012-11-23)
Lamina I of the spinal dorsal horn is a major site of integration and transmission to higher centers of nociceptive information from the periphery. One important primary afferent population that transmits such information to the spinal cord expresses substance P
Neurokinin 1 and opioid receptors: relationships and interactions in nervous system
Xiao, Jie, et al
Translational perioperative and pain medicine, 1, 11-21 (2016)
Expression of Truncated Neurokinin-1 Receptor in Childhood Neuroblastoma is Independent of Tumor Biology and Stage.
Pohl A, et al.
Anticancer Research, 37, 6079-6085 (2017)
Huizhen Huang et al.
Genesis (New York, N.Y. : 2000), 54(11), 593-601 (2016-11-01)
The Neurokinin 1 Receptor (NK1R), which binds Substance P, is expressed in discrete populations of neurons throughout the nervous system, where it has numerous roles including the modulation of pain and affective behaviors. Here, we report the generation of a

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