S8939

SGK1 (δ1-60, S422D), active human

recombinant, expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged), buffered aqueous solution

• MDL number: MFCD03458482
• UNSPSC Code: 51111800
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged)
• Quality Level: 200
• form: buffered aqueous solution
• mol wt: ~48 kDa by SDS-PAGE
• UniProt accession no.: O00141
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SGK1(6446)

Description

General description

The serum- and glucocorticoid-regulated kinase (SGK1) is a member of the AGC kinase family and the gene encoding it is localized on human chromosome 6q23.

Biochem/physiol Actions

The serum- and glucocorticoid-regulated kinase (SGK1) has a role in the hormonal regulation of ion channels and pumps. It has been associated with cancers. The protein modulates nitric oxide production by activating NO synthase. SGK1 protects cells from apoptosis and oxidative stress.

Kinase assays.

Unit Definition

One unit of SGK1 = 1 nmol phosphate incorporated into crosstide substrate peptide in one minute.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 0.15 M NaCl, 0.03% Brij^® L23, 1 mM benzamidine, 0.2 mM PMSF. and 270 mM sucrose.

Legal Information

Brij is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 10 - Combustible liquids