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S9059

Sigma-Aldrich

Streptavidin−Gold from Streptomyces avidinii

~10 nm nominal (colloidal gold), buffered aqueous glycerol solution

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MDL number:
NACRES:
NA.46

conjugate

gold conjugate

Quality Level

form

buffered aqueous glycerol solution

concentration

~2.5 A520 units/mL

nominal

~10 nm (colloidal gold)

mean particle size

8-12 nm (monodisperse)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
S6402S9415S2890
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

-

form

lyophilized powder

concentration

~2.5 A520 units/mL

concentration

-

concentration

-

concentration

-

nominal

~10 nm (colloidal gold)

nominal

-

nominal

-

nominal

-

mean particle size

8-12 nm (monodisperse)

mean particle size

-

mean particle size

-

mean particle size

-

General description

Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.

Application

Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.
For detection of biotinylated compounds.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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