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Key Documents

SAB2700730

Sigma-Aldrich

Monoclonal Anti-TET1 antibody produced in mouse

clone GT1462, affinity isolated antibody, buffered aqueous solution

Synonym(s):

CXXC6, FLJ10839, FLJ41442, TET1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT1462, monoclonal

form

buffered aqueous solution

species reactivity

human, mouse

technique(s)

ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: 1:100-1:1000
immunoprecipitation (IP): suitable
western blot: 500-3000

isotype

IgG2

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TET1(80312)

General description

Tet methylcytosine dioxygenase 1 (TET1) is a protein that belongs to the TET family and contains a zinc-binding CXXC domain. It is expressed in embryonic stem cells. The TET1 gene is localized on human chromosome 10q21.3.

Application

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

Tet methylcytosine dioxygenase 1 (TET1) functions as a 5-methylcytosine (5mC) hydroxylase. It converts 5-methylcytosine to 5-hydroxymethylcytosine, which is crucial for active DNA demethylation in the adult brain. The protein has been linked to the progression of colorectal cancer.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Phosphate-buffered saline, no preservative added.

Storage and Stability

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Other Notes

For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for animal or human consumption.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel isoform of TET1 that lacks a CXXC domain is overexpressed in cancer.
Good CR, et al.
Nucleic Acids Research (2017)
Genomic and epigenomic analysis of high-risk prostate cancer reveals changes in hydroxymethylation and TET1
Lien Spans, et al.
Oncotarget (2016)
Faiza Noreen et al.
Clinical epigenetics, 11(1), 196-196 (2019-12-18)
Aberrations in DNA methylation are widespread in colon cancer (CC). Understanding origin and progression of DNA methylation aberrations is essential to develop effective preventive and therapeutic strategies. Here, we aimed to dissect CC subtype-specific methylation instability to understand underlying mechanisms
Association of TET1 expression with colorectal cancer progression.
Scandinavian Journal of Gastroenterology (2017)
LCX, leukemia-associated protein with a CXXC domain, is fused to MLL in acute myeloid leukemia with trilineage dysplasia having t(10;11)(q22;q23)
Ryoichi Ono, et al
Cancer Research, 2002 (2002)

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