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SAB4200017

Sigma-Aldrich

Anti-MUC1 antibody , Mouse monoclonal

clone 1D1, purified from hybridoma cell culture

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Synonym(s):
Anti-CD227, Anti-EMA, Anti-H23AG, Anti-KL-6, Anti-MAM6, Anti-Mucin1, Anti-PEM, Anti-PEMT, Anti-PUM

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

1D1, monoclonal

form

buffered aqueous solution

mol wt

antigen 160-500 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 0.5-1.0 μg/mL using T47D cell lysates

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MUC1(4582)

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This Item
SAB4501933SAB2101529AV41446
biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

clone

1D1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

dog, human

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General description

Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. Muc1 is a type I transmembrane glycoprotein with a unique extracellular domain containing 20 amino acid variable number tandem repeat (VNTR) domain. In many epithelia, this protein is attached to the apical surface by a transmembrane domain. The degree of glycosylation varies with cell type. The MUC1 gene is mapped to human chromosome 1q22.

Immunogen

semi purified MUC1 from pleural effusion of a breast cancer patient.

Application

Monoclonal Anti-MUC1 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • radioimmunoassay
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

Mucin 1 (Muc1) exhibits protective action by binding to pathogens and is also associated with cell signaling capacity. Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. Muc1 might serve as a candidate for single chain antibody mediated targeted therapy in MUC1 expressing cancers. They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

Purchase of this product conveys a non-exclusive license to use the product for research purposes only. Purchase of this product does not convey a right to (i) incorporate or use the product in the manufacture of any commercial product, (ii) sell or otherwise transfer the product to any third party, or (iii) use the product to provide any commercial service. THE PRODUCT IS NOT FOR IN VIVO HUMANS USE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mucins in cancer: protection and control of the cell surface
Hollingsworth MA and Swanson BJ
Nature Reviews. Cancer, 4(1), 45-45 (2004)
MUC1: a target molecule for cancer therapy
Singh R and Bandyopadhyay D
Cancer Biology & Therapy, 6(4), 481-486 (2007)
The MUC1 oncoprotein as a functional target: immunotoxin binding to alphabeta junction mediates cell killing
Rubinstein DB, et al.
International Journal of Cancer. Journal International Du Cancer, 124(1), 46-54 (2009)
Genetic variation in C20orf54, PLCE1 and MUC1 and risk of upper gastrointestinal cancers in Caucasian populations.
Palmer A J, et al.
European Journal of Cancer Prevention, 21(6), 541-541 (2012)

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