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SAB4200057

Sigma-Aldrich

Anti-Rab13 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-GIG4, Anti-RAS-associated protein RAB13
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~23 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human NT2 cells
indirect immunofluorescence: 2-5 μg/mL using mouse 3T3 cells
western blot: 2.5-5.0 μg/mL using whole extracts of human NT2 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Rab13(68328)

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R0781R0656R4779
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

mol wt

antigen ~23 kDa

mol wt

antigen ~24 kDa

mol wt

antigen ~24 kDa

mol wt

antigen ~23 kDa

UniProt accession no.

P51153

UniProt accession no.

P61106

UniProt accession no.

P61106

UniProt accession no.

P51149

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Rab13 is a member of the Rab family of Ras superfamily of small guanosine triphosphatases (GTPases). Rab13 is localized to the cytoplasmic vesicles of nonpolarized cells and at tight junctions (Tjs) in polarized epithelial cells.

Immunogen

synthetic peptide corresponding to amino acids 46-58 of mouse Rab13, conjugated to KLH. The corresponding sequence differs by a single amino acid in human and rat Rab13.

Application

Anti-Rab13 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Rab guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of a Rab protein is coupled to its association with intracellular membranes. Rab proteins contain conserved regions involved in guanine-nucleotide binding and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Rab13 mediates tight junctions assembly in epithelial cells by regulating cAMP dependent protein kinase (PKA) mediated phosphorylation of vasodilator stimulated phosphoprotein (VASP) and its recruitment to cell-cell junctions. Rab13 also regulates membrane trafficking between the thyroglobulin (TGN) and recycling endosomes in polarized epithelial cells during biosynthetic delivery. Each Rab protein shows a characteristic subcellular distribution. Therefore, antibodies to Rab proteins may serve as useful tools for studying subcellular localization and membrane organization.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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The Anaplasma phagocytophilum-occupied vacuole selectively recruits Rab-GTPases that are predominantly associated with recycling endosomes
Huang B, et al.
Cellular Microbiology, 12(9), 1292-1307 (2010)
Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells
Zhang Z, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1864, 613-624 (2017)
Rab proteins as membrane organizers
Zerial M and McBride H
Nature Reviews in Molecular and Cell Biology, 2(2), 107-107 (2001)
Multiple regions contribute to membrane targeting of Rab GTPases
Ali BR, et al.
Journal of Cell Science, 117(26), 6401-6412 (2004)
Rita L Nokes et al.
The Journal of cell biology, 182(5), 845-853 (2008-09-10)
To maintain polarity, epithelial cells continuously sort transmembrane proteins to the apical or basolateral membrane domains during biosynthetic delivery or after internalization. During biosynthetic delivery, some cargo proteins move from the trans-Golgi network (TGN) into recycling endosomes (RE) before being

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