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SAB4200338

Sigma-Aldrich

Anti-KLF5 antibody , Mouse monoclonal

clone KLF5-55, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-BTEB2, Monoclonal Anti-CKLF, Monoclonal Anti-IKLF, Monoclonal Anti-KLF5, Monoclonal Anti-Kruppel-like factor 5 (intestinal)
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

KLF5-55, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55

species reactivity

rat, mouse, chicken, human, hamster, canine, monkey

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using methanol/acetone fixed PC3 cells.
western blot: 1.0-2.0 μg/mL using extracts of PC3 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KLF5(688)
mouse ... Klf5(12224)
rat ... Klf5(84410)

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SAB4200465SAB4200360SAB4200416
species reactivity

rat, mouse, chicken, human, hamster, canine, monkey

species reactivity

mouse, human

species reactivity

human, mouse, rat

species reactivity

human

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

Gene Information

human ... KLF5(688)
mouse ... Klf5(12224)
rat ... Klf5(84410)

Gene Information

human ... FERMT1(55612)
mouse ... Fermt1(241639)

Gene Information

human ... PPIB(5479)
mouse ... Ppib(19035)
rat ... Ppib(64367)

Gene Information

human ... TNS3(64759)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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General description

Kruppel like factor 5 (KLF5) is encoded by the gene mapped to human chromosome 13q22.1. The encoded protein belongs to kruppel-like factor (KLF) protein family and is mainly expressed in variety of tissues, including lung, colon, intestine and pancreas.
Monoclonal Anti-KLF5 (mouse IgG1 isotype) is derived from the hybridoma KLF-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. KLF5 is a DNA-binding transcriptional regulator. This protein contains three independent peptide modules of the C2H2 zinc finger-type. KLF5 protein is expressed in the prostate, kidney, skeletal muscle, and breast. KLF5 gene is also expressed in embryos and is highly expressed in the later stage of fetal development.

Specificity

Monoclonal Anti-KLF5 recognizes human, monkey, dog, hamster, mouse and rat KLF5.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human KLF5, conjugated to KLH. The corresponding sequence is identical to monkey, bovine, chicken, rat and mouse KLF5.

Application

Anti-KLF5 antibody, Mouse monoclonal has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

KLF5 protein functions through the restoration of cyclin D1, cyclin B1 and Cdc2 gene expression. This protein is also involved in regulating the inhibitory effect of retinoids on cell proliferation. KLF5 protein regulates the pro-proliferative and transforming activities of oncogenic H-ras. KLF5 protein plays a role as a tumor suppressor in intestinal tumorigenesis in vivo. Deletion and down-regulation of KLF5 leads to prostate and breast cancer.
Kruppel like factor 5 (KLF5) has been involved in the regulation of various cellular functions, such as cell cycle, apoptosis, proliferation and differentiation, in both normal and tumor cells. Additionally, it also plays a vital role in maintenance of cardiovascular remodeling. Overexpression of the gene has been observed in various cancer types, including cervical cancer(CC) and colon cancer. Hence, this can be used as a prognostic marker for these cancers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dan Tong et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(8), 2442-2448 (2006-04-28)
Kruppel-like factor (KLF5) is a cell growth mediator in various epithelial cells. Higher KLF5 increases cell growth rate and leads to transformed phenotypes. Because tumor cell proliferation is tightly associated with tumor progression, and consequently, with survival of cancer patients
Characterization of the expression profiles of adipogenesis-related factors, ZNF423, KLFs and FGF10, during preadipocyte differentiation and abdominal adipose tissue development in chickens
Matsubara Y
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 165, 189-195 (2013)
KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1?
Li X
International Journal of Oncology, 45, 1507-1514 (2014)
FBW7-mediated ubiquitination and degradation of KLF5
Yi Luan
World Journal of Biological Chemistry, 5, 216-223 (2014)
Weishi Wang et al.
Poultry science, 96(1), 200-210 (2016-09-03)
Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the

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