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SAB4200465

Sigma-Aldrich

Anti-Kindlin-1 antibody, Mouse monoclonal

clone KN-4, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-C20orf42, Monoclonal Anti-DTGCU2, Monoclonal Anti-FERMT1 URP1, Monoclonal Anti-KIND1, Monoclonal Anti-Kindlin-1 antibody produced in mouse, Monoclonal Anti-UNC112A, Monoclonal Anti-fermitin family member 1
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

KN-4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa (isoform 4)
antigen ~60 kDa (isoform 2)
antigen ~77 kDa (isoform 1)

species reactivity

mouse, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using SW-620 cells
western blot: 1.0-2.0 μg/mL using SW-48 total cell extracts

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FERMT1(55612)
mouse ... Fermt1(241639)

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This Item
SAB4200338SAB4200479SAB4200365
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

KN-4, monoclonal

clone

KLF5-55, monoclonal

clone

SET51, monoclonal

clone

PIWIL1-2, monoclonal

species reactivity

mouse, human

species reactivity

rat, mouse, chicken, human, hamster, canine, monkey

species reactivity

rat, human, mouse

species reactivity

human, mouse

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

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General description

Kindlin-1 is coded by FERMT1 (fermitin family member 1) gene. It is a focal adhesion protein, expressed in the skin and intestine. FERMT1 is located on human chromosome 20p.12.3.

Immunogen

synthetic peptide corresponding to a sequence close to the N-terminus of human Kindlin-1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Kindlin-1 antibody has been used in immunohistochemistry, immunofluorescence staining and western blotting.

Biochem/physiol Actions

Kindlin-1 protein, coded by FERMT1 gene modulates keratinocyte electrotaxis. It participates in the initiation of the integrin family of extracellular matrix receptors. FERMT1 maintains the lamellipodial protrusions at the time of electrotaxis. Mutations in kindlin-1 results in Kindler syndrome (KS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Case of Kindler syndrome resulting from mutation in the FERMT1 gene
Wada MM, et al.
The Journal of Dermatology, 39(12), 1057-1058 (2012)
Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing
Shen CS, et al.
International Journal of Molecular Medicine, 39(4), 949-959 (2017)
Kindlin-2 controls TGF-beta signalling and Sox9 expression to regulate chondrogenesis
Wu CJ, et al.
Nature Communications, 6(4), 7531-7531 (2015)
Congcong Shen et al.
International journal of molecular medicine, 39(4), 949-959 (2017-03-16)
The commercial use of epidermal growth factor (EGF) is extensive and has been shown to be effective for skin wound healing in clinical practice. There is evidence to indicate that the topical administration of EGF significantly accelerates re-epithelialization by promoting keratinocyte
New intragenic and promoter region deletion mutations in FERMT 1 underscore genetic homogeneity in Kindler syndrome
Fuchs-Telem D, et al.
Clinical and Experimental Dermatology, 39(3), 361-367 (2014)

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