MilliporeSigma
All Photos(1)

Documents

SAB4200548

Sigma-Aldrich

Anti-IDH1 (R132H) antibody, Mouse monoclonal

clone HMab-1, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Idh1 R132H Antibody, Idh1 R132H Antibody - Anti-IDH1 (R132H) antibody, Mouse monoclonal, Anti-IDCD, Anti-IDH, Anti-IDP, Anti-IDPC, Anti-Isocitrate dehydrogenase 1 (NADP+), soluble, Anti-NADP(+)-specific ICDH, Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Anti-NADP-dependent isocitrate dehydrogenase, peroxisomal, Anti-Oxalosuccinate decarboxylase, Anti-PICD

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HMab-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1R132H.

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IDH1(3417)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200522SAB3500511SAB4200592
Gene Information

human ... IDH1(3417)

Gene Information

human ... IDH1(3417)
mouse ... Idh1(15926)
rat ... Idh1(24479)

Gene Information

human ... IDH1(3417)

Gene Information

human ... IDH1(3417)

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

human, rat, mouse

species reactivity

mouse, human

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.

Immunogen

peptide corresponding to mutation R132H of human IDH1.

Application

Anti-IDH1 (R132H) antibody has been used:
  • in immunoblotting
  • in immunohistochemistry
  • in western blotting
  • in cell cycle analysis and apoptosis assays
  • in chromatin immunoprecipitation (ChIP) assay
  • in in vitro migration assay

Biochem/physiol Actions

Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

Dimethyl sulfoxide Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sigma-Aldrich

D2650

Dimethyl sulfoxide

Shuchen Chen et al.
Cancer medicine, 11(22), 4122-4133 (2022-05-09)
Isocitrate dehydrogenase (IDH) is an appealing target for anticancer therapy, and IDH (IDH1/2) inhibitors have been approved for targeted therapy of acute myeloid leukemia (AML) and Cholangiocarcinoma. The therapeutic potential of IDH inhibitors for non-small-cell lung cancer (NSCLC) patients is
Clinicopathological Analysis of HIF-1alpha and TERT on Survival Outcome in Glioblastoma Patients: A Prospective, Single Institution Study
Potharaju M, et al.
Journal of Cancer, 10(11), 2397-2406 (2019)
Molecular mechanisms of isocitrate dehydrogenase 1 (IDH1) mutations identified in tumors: the role of size and hydrophobicity at residue 132 on catalytic efficiency
Matteo D A, et al.
The Journal of biological chemistry, 292(19), 7971-7983 (2017)
Bin Sheng Wong et al.
Nature biomedical engineering, 5(1), 26-40 (2020-09-30)
Clinical scores, molecular markers and cellular phenotypes have been used to predict the clinical outcomes of patients with glioblastoma. However, their clinical use has been hampered by confounders such as patient co-morbidities, by the tumoral heterogeneity of molecular and cellular
Mahadev Potharaju et al.
Journal of Cancer, 10(11), 2397-2406 (2019-07-02)
Glioblastoma multiforme is a highly malignant and aggressive primary brain tumor with a dismal prognosis. We studied the association of immunohistochemical expression of hypoxia inducible factor-1 alpha (HIF-1α), telomerase reverse transcriptase (TERT), isocitrate dehydrogenase 1 (IDH1) and tumor protein p53

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service