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SAB4200683

Sigma-Aldrich

Anti-Human IgG4 antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Human IgG4 antibody produced in mouse
NACRES:
NA.46

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HP-6025, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: suitable
indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml human IgG4 myeloma proteins for coating.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
SAB4200713I7385SAB4200788
antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

clone

HP-6025, monoclonal

clone

GG-5, monoclonal

clone

HP-6025, monoclonal

clone

HP-6001, monoclonal

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Monoclonal Anti-Human IgG4 (mouse IgG1 isotype) is derived from the HP-6025 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG4 is a subclass of IgG with γ4 heavy chain.

Specificity

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Immunogen

Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.

Biochem/physiol Actions

IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiectasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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