SAB4200732

Anti-β-Tubulin I antibody, Mouse monoclonal

clone SAP.4G5, purified from hybridoma cell culture

• Synonym(s): Anti-TUBB
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SAP.4G5, monoclonal
• form: buffered aqueous solution
• mol wt: ~55 kDa
• species reactivity: bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract; immunofluorescence: 1-2.5 μg/mL using HeLa cells; immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.
• isotype: IgG1
• UniProt accession no.: P07437
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB(203068)

Description

General description

Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.

Immunogen

synthetic peptide corresponding to the C-terminal sequence of ß-tubulin isotype I, conjugated to BSA

Application

Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable