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SAB4200744

Sigma-Aldrich

Anti-BDNF antibody, Mouse Monoclonal

clone BDNF129-13, purified from hybridoma cell culture

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Synonym(s):
Anti-Abrineurin, Anti-Brain-derived neurotrophic factor
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BDNF129-13, monoclonal

form

buffered aqueous solution

mol wt

~12 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein
immunocytochemistry: suitable
immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections
immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BDNF(627)

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This Item
P1374E8159SAB4200337
biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

mouse, human

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

BDNF129-13, monoclonal

clone

polyclonal

clone

EDEM1-17, monoclonal

clone

p-RasGRP1-1, monoclonal

Gene Information

human ... BDNF(627)

Gene Information

human ... BDNF(627)
mouse ... Bdnf(12064)
rat ... Bdnf(24225)

Gene Information

human ... EDEM1(9695)

Gene Information

human ... RASGRP1(10125)
mouse ... Rasgrp1(19419)
rat ... Rasgrp1(29434)

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General description

Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).

Immunogen

Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH

Application

Anti-BDNF antibody, Mouse Monoclonal has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Patricia Sampedro-Piquero et al.
Addiction biology, 27(2), e13158-e13158 (2022-03-02)
Our aim was to assess the cognitive and emotional state, as well as related-changes in the glucocorticoid receptor (GR), the corticotropin-releasing factor (CRF) and the brain-derived neurotrophic factor (BDNF) expression of adolescent C57BL/6J male mice after a 5-week two-bottle choice
Brain-derived neurotrophic factor and its clinical implications
Bathina S and Das UN
Archives of Medical Science : Ams, 11(6), 1164-1164 (2015)
Peripheral vascular reactivity and serum BDNF responses to aerobic training are impaired by the BDNF Val66Met polymorphism
Lemos Jr J R, et al.
Physiological Genomics, 48, 116-123 (2015)
The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function
Egan M F, et al.
Cell, 257-269 (2003)
Electroacupuncture at the Baihui acupoint alleviates cognitive impairment and exerts neuroprotective effects by modulating the expression and processing of brain-derived neurotrophic factor in APP/PS1 transgenic mice.
Lin R
Molecular Medicine Reports, 13(2), 1611-1617 (2016)

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