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SAB4200757

Sigma-Aldrich

Anti-PLA2R antibody, Mouse monoclonal

clone 12-6-5, purified from hybridoma cell culture

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Synonym(s):
Anti-180 kDa secretory phospholipase A2 receptor, Anti-C-type lectin domain family 13 member C, Anti-M-type receptor, Anti-PLA2-R, Anti-PLA2R, Anti-Secretory phospholipase A2 receptor

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

12-6-5, monoclonal

form

buffered aqueous solution

mol wt

~180 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable
immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa)
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PLA2R1(22925)

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This Item
SAB4200412AMAB90772MABC942
Anti-PLA2R Antibody, clone 5F5.1 clone 5F5.1, from mouse

MABC942

Anti-PLA2R Antibody, clone 5F5.1

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

12-6-5, monoclonal

clone

PL231, monoclonal

clone

CL0474, monoclonal

clone

5F5.1, monoclonal

species reactivity

human

species reactivity

monkey, rat, canine, human, mouse

species reactivity

human

species reactivity

human

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa), immunohistochemistry: suitable

technique(s)

indirect immunofluorescence: suitable, western blot: 4.0-8.0 μg/mL using extracts of HL-60 cells

technique(s)

immunoblotting: 1 μg/mL, immunofluorescence: 2-10 μg/mL, immunohistochemistry: 1:10000-1:20000

technique(s)

immunohistochemistry: suitable, western blot: suitable

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General description

Anti-PLA2R antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the 12-6-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with recombinant fragment of human PLA2R corresponding to extracellular sequence NC3. Secretory phospholipase A2 receptor (PLA2R) is 180 kDa and is a type I transmembrane glycoprotein member of the mannose receptor (MR) family. Large extracellular glycosylated region comprising an N-terminal cysteine-rich domain (CysR), a fibronectin-like type II domain (FnII), and eight to ten C-type lectin-like domains (CTLD).

Immunogen

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3)

Application

Anti-PLA2R antibody has been used in
  • immunoblotting
  • immunofluorescence
  • flow Cytometry
  • immunohistochemistry

Biochem/physiol Actions

Secretory phospholipase A2 receptor (PLA2R) acts as a receptor for secretory phosholipase A2 (sPLA2) allowing its removal from circulation, thus regulating its biological effect. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipase A2 (sPLA2) from the blood, including group sPLA2-X and a snake venom sPLA2. The soluble circulating form of PLA2R is constitutively present as an endogenous inhibitor of sPLA2s. PLA2R is expressed in kidney podocytes of normal human glomeruli, in idiopathic membranous nephropathy (IMN) patients PLA2R is colocalized to immune deposits in glomeruli along with IgG4.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Phospholipase A2 receptor: a regulator of biological functions of secretory phospholipase A2
Hanasaki, Kohji and Arita, Hitoshi
Prostaglandins & other lipid mediators, 68, 71-82 (2002)
Antiphospholipase A2 receptor autoantibodies: a step forward in the management of primary membranous nephropathy
Obrisca B, et al.
BioMed Research International, 2015(3) (2015)
M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy
Beck Jr L H, et al.
The New England Journal of Medicine, 361(1), 11-21 (2009)
Membranous nephropathy: integrating basic science into improved clinical management
Cattran D C and Brenchley P E
Kidney International, 91(3), 566-574 (2017)

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