SAB4200762

Anti-Opsin antibody, Mouse monoclonal

clone RET-P1, purified from hybridoma cell culture

• Synonym(s): Anti-Opsin 2, Anti-Rhodopsin
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: RET-P1, monoclonal
• form: buffered aqueous solution
• mol wt: ~39 kDa
• species reactivity: avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit
• concentration: ~1 mg/mL
• technique(s): ELISA: suitable; immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract. ; immunofluorescence: suitable; immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.; radioimmunoassay: suitable
• isotype: IgG1
• UniProt accession no.: P08100
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Rho(24717)

Description

General description

Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.

Application

Anti-Opsin antibody has been used in

• immunoblotting
• immunofluorescence
• immunohistochemistry
• enzyme linked immunosorbent assay (ELISA)
• radioimmuno assay (RIA)

Biochem/physiol Actions

Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.

Other Notes

rat retinal membranes

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable