MilliporeSigma
All Photos(1)

Documents

SAB4200782

Sigma-Aldrich

Anti-Tenascin antibody, Mouse monoclonal

clone BC-24, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-Cytotactin, Anti-GMEM, Anti-GP 150-225, Anti-Glioma-associated-extracellular matrix antigen, Anti-Hexabrachion, Anti-JI, Anti-Myotendinous antigen, Anti-Neuronectin, Anti-TN, Anti-Tenascin-C (TN-C)
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BC-24, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunoblotting: suitable
immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNC(3371)

Related Categories

General description

Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa.

Immunogen

Human tenascin

Application

Anti-Tenascin antibody has been used:
  • in immunoblotting
  • in Immunohistochemistry
  • in flow cytometry
  • in enzyme linked immunosorbent assay(ELISA)
  • for blocking tenascin-C proliferating activity

Biochem/physiol Actions

Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service