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Key Documents

SAB4500028

Sigma-Aldrich

Anti-AOS1 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

AOS1, SAE1, SUA1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 38 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
immunofluorescence: 1:100-1:500
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SAE1(10055)

Related Categories

General description

E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle.

Immunogen

The antiserum was produced against synthesized peptide derived from human AOS1.

Immunogen Range: 221-270

Biochem/physiol Actions

SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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SUMO-activating SAE1 transcription is positively regulated by Myc.
Amente S, et al.
American Journal of Cancer Research, 2(3), 330-334 (2012)
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, et al.
Faseb Journal, 15(10), 1825-1827 (2001)
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.
Tatham MH, et al.
The Journal of Biological Chemistry, 276(38), 35368-35374 (2001)
Robert Coukos et al.
eLife, 10 (2021-08-21)
The trafficking of specific protein cohorts to correct subcellular locations at correct times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach to probe the molecular mechanisms of protein trafficking, but

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