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SAB4502573

Sigma-Aldrich

Anti-C-RAF antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
C-RAF, C-Raf, CRAF, Protein kinase raf 1, kinase Raf1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 73 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAF1(5894)

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This Item
SAB4502578SAB4502576SAB4502577
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

UniProt accession no.

P04049

UniProt accession no.

P04049

UniProt accession no.

P04049

UniProt accession no.

P04049

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Anti-C-RAF Antibody detects endogenous levels of total C-RAF protein.
Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is an anti-apoptotic protein. The gene encoding it is localized on human chromosome 3p25.2.

Immunogen

The antiserum was produced against synthesized peptide derived from human C-RAF.

Immunogen Range: 271-320

Biochem/physiol Actions

Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is a crucial component of the Raf/mitogen-activated extracellular signal-regulated kinase (MEK)/extracellular regulated protein kinase (ERK) signaling cascade. It functions as an activator of MEK-1 and -2. The protein is upregulated in many cancers. Mutations in the gene encoding RAF1 have been linked to Noonan syndrome.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Neonatal pulmonary arterial hypertension and Noonan syndrome: two fatal cases with a specific RAF1 mutation.
Hopper RK
American Journal of Medical Genetics (2015)
Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy
Bhaswati Pandit
Nature Genetics (2007)
EZH2-mediated epigenetic repression of DNA repair in promoting breast tumor initiating cells
Breast Cancer Research (2011)
The lack of Raf-1 kinase feedback regulation enhances antiapoptosis in cancer cells.
Ma SQ
Oncogene (2017)
Overexpression of Raf-1 and ERK1/2 in sacral chordoma and association with tumor recurrence.
International Journal of Clinical and Experimental Pathology (2015)

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