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SAB4504331

Sigma-Aldrich

Anti-phospho-Akt (pSer473) antibody produced in rabbit

affinity isolated antibody

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NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer473)

Gene Information

human ... AKT1(207)
rat ... Akt1(24185)

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SAB4504014SAB4500802SAB4504017
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

species reactivity

human, mouse, rat

species reactivity

human, mouse, rat

species reactivity

human, rat, mouse

species reactivity

rat, human, mouse

NCBI accession no.

P31749, Q9Y243, P31751

NCBI accession no.

P31749

NCBI accession no.

P31749

NCBI accession no.

P31749

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General description

AKT1 (AKT serine/threonine kinase 1) is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is expressed in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α helical linker and a carboxyl-terminal kinase domain.

Immunogen

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473.

Immunogen Range: 431-480

Application

Anti-phospho-Akt (pSer473) antibody has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

AKT1 (AKT serine/threonine kinase 1) controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers , hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shih-Ron Hsieh et al.
Journal of biomedical science, 20, 86-86 (2013-11-21)
Epigallocatechin-3-gallate (EGCg) with its potent anti-oxidative capabilities is known for its beneficial effects ameliorating oxidative injury to cardiac cells. Although studies have provided convincing evidence to support the cardioprotective effects of EGCg, it remains unclear whether EGCg affect trans-membrane signalling
Epigallocatechin-3-gallate-mediated cardioprotection by Akt/GSK-3?/caveolin signalling in H9c2 rat cardiomyoblasts
Hsieh SR, et al.
Journal of Biomedical Science (2013)
Association between single nucleotide polymorphisms in AKT1 and the risk of prostate cancer in the Chinese Han population
Liu JM, et al.
Genetics and molecular research : GMR, 16(1) (2017)
Hongfang Li et al.
OncoTargets and therapy, 10, 5685-5694 (2017-12-12)
Pre-B-cell leukemia homeobox 3 (PBX3) is upregulated in various malignancies; however, the role of PBX3 in cervical cancer (CC) is unknown. The purpose of this study was to explore the expression characteristics, clinicopathological significance, and molecular biological function of PBX3
J Matthew Kuczmarski et al.
Experimental physiology, 103(4), 545-558 (2018-01-10)
What is the central question of this study? Translocation of nNOSμ initiates catabolic signalling via FoxO3a and skeletal muscle atrophy during mechanical unloading. Recent evidence suggests that unloading-induced muscle atrophy and FoxO3a activation are redox sensitive. Will a mimetic of

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