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SAB5701044

Sigma-Aldrich

Anti-Sonic Hedgehog (Shh) antibody produced in rabbit

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biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

monoclonal

form

liquid

species reactivity

human, mouse, rat

concentration

0.45 mg/mL

technique(s)

immunohistochemistry: 1:50-1:200
western blot: 1:500-1:2000

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SHH(6469)

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This Item
S8321SAB1300534S9822
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

goat

clone

monoclonal

clone

SH154, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... SHH(6469)

Gene Information

human ... SHH(6469)
mouse ... Shh(20423)
rat ... Shh(29499)

Gene Information

human ... SHB(6461)

Gene Information

mouse ... Shh(20423)

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections, indirect ELISA: 2 μg/mL, western blot: 2 μg/mL using recombinant mouse SHH peptide

technique(s)

immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000

technique(s)

immunohistochemistry: 15 μg/mL, western blot: 0.1-0.2 μg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Immunogen

A synthesized peptide derived from human Sonic Hedgehog (Shh) (Shh)

Physical form

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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