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SAE0212

Sigma-Aldrich

Mucin Probe, StcE

Biotin and FLAG® tagged, powder

Synonym(s):

Catalytically inactive Mucin specific protease StcE

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

200

form

powder

UniProt accession no.

O82882

shipped in

wet ice

storage temp.

-10 to -25°C

General description

Mucin Probe, StcE is a proteolytically inactive mutant (E447D) form of Mucinase StcE that maintains ability to tightly bind mucin proteins. The recombinant protein carries a 3XFLAG tag and is also enzymatically biotinylated at the C-term of the protein. While there are many applications, this product is designed to probe mucin dense tissues. It will non-covalently, and selectively bind to Mucin-domain glycoproteins with dense O-linked glycosylation. Secondary reporting tools can then be used to bind the probe using its FLAG® tag or Biotin conjugate.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000250763
0000225525

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Keira E Mahoney et al.
Current protocols, 4(7), e1100-e1100 (2024-07-10)
Mucin-domain glycoproteins are characterized by their high density of glycosylated serine and threonine residues, which complicates their analysis by mass spectrometry. The dense glycosylation renders the protein backbone inaccessible to workhorse proteases like trypsin, the vast heterogeneity of glycosylation often

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