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SBPE06

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SB-ratMdr1b-Sf9-PREDEASY-ATPase kit

membrane based kit for Rat Mdr1b ATPase Transport Assays

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Synonym(s):
Mdr1b, Mdr1b Rat membrane based kit for Rat Mdr1b ATPase Transport Assays, P-gp, multidrug resistance protein 1b, p-glycoprotein, rat Abcb1b

recombinant

expressed in baculovirus infected Sf9 cells
expressed in insect cells

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

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SBPE01SBPE03SBPE13
shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Gene Information

rat ... ABCB1B(24646)

Gene Information

human ... ABCB1(5243)

Gene Information

human ... ABCC2(1244)

Gene Information

-

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in insect cells

recombinant

expressed in insect cells

recombinant

expressed in insect cells

General description

SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Application

SOLVO′s PREDEASY ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.

PREDIVEZ PREDEASY Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.

PREDEASY ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.

Legal Information

Distributed for SOLVO Biotechnology, Inc.
PREDEASY is a trademark of Solvo Biotechnology
PREDIVEZ is a trademark of Solvo Biotechnology

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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MDR1, the blood-brain barrier transporter, is associated with Parkinson's disease in ethnic Chinese.
C G L Lee et al.
Journal of medical genetics, 41(5), e60-e60 (2004-05-04)
Drug-drug interactions of new active substances: mibefradil example.
M Siepmann et al.
European journal of clinical pharmacology, 56(3), 273-273 (2000-08-22)
Zsuzsanna Rajnai et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(11), 2000-2006 (2010-08-12)
Seliciclib, a cyclin-dependent kinase inhibitor, is a promising candidate to treat a variety of cancers. Pharmacokinetic studies have shown high oral bioavailability but limited brain exposure to the drug. This study shows that seliciclib is a high-affinity substrate of ATP-binding
Siegfried Drescher et al.
British journal of clinical pharmacology, 53(5), 526-534 (2002-05-08)
The C3435T polymorphism in the human MDR1 gene is associated with lower intestinal P-glycoprotein expression, reduced protein function in peripheral blood cells and higher plasma concentrations of the P-glycoprotein substrate digoxin. Using fexofenadine, a known P-glycoprotein substrate, the hypothesis was
R B Wang et al.
Journal of clinical pharmacy and therapeutics, 28(3), 203-228 (2003-06-11)
A large number of structurally and functionally diverse compounds act as substrates or modulators of p-glycoprotein (p-gp). Some of them possess multiple drug resistance (MDR)-reversing activity, but only a small number of them have entered clinical study. In order to

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