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SBR00013

Sigma-Aldrich

Actinomycin D, Ready Made Solution

from Streptomyces sp., 2 mg/mL in DMSO

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Synonym(s):
Actinomycin D solution from Streptomyces sp., Actinomycin IV, Dactinomycin
Empirical Formula (Hill Notation):
C62H86N12O16
CAS Number:
Molecular Weight:
1255.42
PubChem Substance ID:

biological source

Streptomyces sp.

Quality Level

description

Ready Made Solution

form

liquid

storage condition

protect from light

concentration

2 mg/mL in DMSO

color

dark orange

antibiotic activity spectrum

neoplastics

mode of action

DNA synthesis | interferes

storage temp.

−20°C

InChI

1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)

InChI key

RJURFGZVJUQBHK-UHFFFAOYSA-N

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This Item
A4262A9415SBR00014
Actinomycin D from Streptomyces sp., ≥95% (HPLC)

A4262

Actinomycin D

Actinomycin D from Streptomyces sp., suitable for cell culture, ≥95%

A9415

Actinomycin D

Daptomycin Ready Made Solution 1 mg/mL in DMSO

SBR00014

Daptomycin Ready Made Solution

mode of action

DNA synthesis | interferes

mode of action

DNA synthesis | interferes

mode of action

DNA synthesis | interferes

mode of action

DNA synthesis | interferes, protein synthesis | interferes

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

liquid

form

powder

form

powder

form

liquid

concentration

2 mg/mL in DMSO

concentration

-

concentration

-

concentration

1 mg/mL in DMSO

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Biochem/physiol Actions

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation. It is a cytotoxic inducer of apoptosis against tumor cells. The compound inhibits the proliferation of cells in a nonspecific way by forming a stable complex with double-stranded DNA (via deoxyguanosine residues), thus inhibiting DNA-primed RNA synthesis. It also causes single strand breaks in DNA.

Actinomycin D has been shown to be an inhibitor of the minus-strand transfer step in reverse transcription, and is therefore used in studies and suppression of HIV-replication. It has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II.

In cell culture, actinomycin D is used as a selection agent. Actinomycin D binding to yeast in ribosomal RNA has been studied. The sensitivity of various strains of E. coli to actinomycin D and the mechanism of binding has been studied. Actinomycin D is also used in molecular biology as a DNA intercalator which blocks the progression of RNA polymerases.

Packaging

1ML

Caution

Actinomycin D solutions are very sensitive to light.

Preparation Note

The 2 mg/mL solution may be further diluted in an appropriate buffer prior to use.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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Goodman and Gilman?s The Pharmacological Basis of Therapeutics, 1240-1241 (1990)
J Kleeff et al.
International journal of cancer, 86(3), 399-407 (2000-04-13)
Pancreatic cancer cells are usually resistant to apoptosis induced by cytotoxic drugs, by activation of surface receptors such as Fas and TNF receptor or by serum or growth factor withdrawal. Actinomycin D (actD) is an inhibitor of RNA synthesis and
Dawson, R.M.C., et al., Eds.
Data for Biochemical Research, 256-257 (1986)
Olivier Bensaude
Transcription, 2(3), 103-108 (2011-09-17)
This review first discusses ways in which we can evaluate transcription inhibition, describe changes in nuclear structure due to transcription inhibition, and report on genes that are paradoxically stimulated by transcription inhibition. Next, it summarizes the characteristics and mechanisms of
Reynolds, J.E.F, Ed.
Martindale: The Extra Pharmacopoeia, 473-473 (1993)

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