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SRP0102

Sigma-Aldrich

HDAC 2 His tag human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

HD2, Histone deacetylase 2, Transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

60 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC2(3066)

General description

Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal His tag, MW = 60 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20, and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

TWEEN is a registered trademark of Croda International PLC

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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