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SRP0227

Sigma-Aldrich

SMURF1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

E3 ubiquitin ligase SMURF1, KIAA1625, SMAD specific E3 ubiquitin protein ligase 1

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W381306-SAMPLE-K
₩81,200
1 KG
₩107,387
10 KG
₩499,685

₩81,200


Please contact Customer Service for Availability


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W381306-SAMPLE-K
₩81,200
1 KG
₩107,387
10 KG
₩499,685

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

₩81,200


Please contact Customer Service for Availability

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥0.0055 pmol/min-μg

mol wt

70 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

General description

Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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LncRNA LITATS1 suppresses TGF-I?-induced EMT and cancer cell plasticity by potentiating TI?RI degradation.
Fan, et al.
The Embo Journal, 42, e112806-e112806 (2023)

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