SRP2004

TFIIE α, p56 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(s): FE, TF2E1, TFIIE-A
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~51.5 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 200 μg/mL
• technique(s): western blot: suitable
• color: clear colorless
• NCBI accession no.: NM_005513
• UniProt accession no.: P29083
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2E1(2960)

Description

Biochem/physiol Actions

The human Transcription Factor IIE (TFIIE) is composed of 56 kDa ( α) and 34 kDa ( β) subunits and is shown to be a heterotetramer. The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable