SRP3326
BMP-2 human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
BMP-2A
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.
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biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
26.0 kDa
packaging
pkg of 10 μg
impurities
endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
General description
The functional form of BMP-2 (bone morphogenetic protein 2) is a 26kDa protein composed of two identical 114 amino-acid polypeptide chains (monomers) linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino-acid signal sequence for secretion, and a 259 amino-acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.[1] Recombinant human BMP-2 is a 26kDa homodimeric disulfide-linked protein consisting of two identical 115 amino acid chains.
Application
BMP-2 human has been used to study the biological role of BMP-2 on adult renal progenitor cells under in vitro and in vivo conditions.[2]
Biochem/physiol Actions
BMP-2 (bone morphogenetic protein 2) is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite.[3] During bone repair, it controls the expression of Runt-related transcription factor 2 (Runx2) and Osterix (Osx), thereby enhancing migration, proliferation and osteogenic differentiation of mesenchymal stem cells.[2] In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues including lung, liver, spleen, prostate, ovary, and small intestine.[4]
Physical form
Lyophilized from 0.1% TFA.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bone morphogenetic proteins: multifunctional regulators of vertebrate development.
Hogan BL
Genes & Development, 10, 1580-1594 (1996)
A role for bone morphogenetic proteins in the induction of cardiac myogenesis.
Schultheiss TM, et al.
Genes & Development, 11, 451-462 (1997)
Vera Hintze et al.
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Sulfated glycosaminoglycans (GAGs) can direct cellular processes by interacting with proteins of the extracellular matrix (ECM). In this study we characterize the interaction profiles of chemically sulfated hyaluronan (HA) and chondroitin sulfate (CS) derivatives with bone morphogenetic protein-2 (BMP-2) and
Bone regeneration with low dose BMP-2 amplified by biomimetic supramolecular nanofibers within collagen scaffolds.
Lee SS, et al.
Biomaterials, 34, 452-459 (2013)
Nam Hee Kim et al.
Biomaterials, 35(37), 9888-9896 (2014-09-15)
Though growth factors allow tissue regeneration, the trade-off between their effectiveness and adverse effects limits clinical application. The key issues in current growth factor therapy largely derive from initial burst pharmacokinetics, rapid clearance, and proteolytic cleavage resulting in clinical ineffectiveness
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