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T0256

Sigma-Aldrich

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

Synonym(s):

BPTI

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202

biological source

bovine pancreas

type

Type I-P

form

essentially salt-free, lyophilized powder

mol wt

~6500 Da (single-chain 58-amino acid peptide)

purified by

crystallization

technique(s)

inhibition assay: suitable

solubility

water: 10 g/L

storage temp.

−20°C

Biochem/physiol Actions

A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K+ channels. It also binds to an intracellular site associated with the large conductance Ca2+-activated K+ channel..

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Preparation Note

Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).

Analysis Note

One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.
Protein determined by biuret.

Other Notes

View more information on Trypsin Inhibitor.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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