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T3952

Sigma-Aldrich

Anti-β-Tubulin III antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Tuj 1
MDL number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

western blot: 1:500-1:1,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

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General description

Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have several different isotypes . β-Tubulin III is a marker for nerve cell differentiation, as well as for venous and lymphatic valves . Changes in β-tubulin III expression have been associated with medulloblastoma
Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Anti-β-Tubulin III antibody is specific for β-Tubulin III in humans.
Tubulin β 3 class III (TUBB3) is mapped to human chromosome 16q24.3.

Immunogen

synthetic peptide corresponding to residues 151-165 of human β-Tubulin III.

Application

Anti-β-Tubulin III antibody is suitable for use in flow cytometry (1:200-1:500), immunohistochemistry (1:100-1:200), immunoblot (approx. 50kDa) and western blot (1:500-1:1,000).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Missense mutations in the tubulin β 3 class III gene (TUBB3) impacts microtubule organisation in neuronal cells and contributes to neurological disorders. Mutations in TUBB3 is implicated in tubulopathies contributing to malformations in developing fetus.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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