Recommended Products
concentration
100 ng/μL
shipped in
wet ice
storage temp.
−20°C
General description
The TargeTron Vector pNL9164 is a 9,295 bp expression vector for use with the TargeTron Gene Knockout System (product number TA0100). This circularized vector can be used for targeted gene knockouts in gram-positive organisms such as Staphyloccus aureus. As supplied, the pNL9164 vector is re-targeted to the S. aureus hsa gene. The hsa gene encodes a member of the HU family of bacterial histone-like proteins. For validation in S. aureus, this vector can be used directly to knockout hsa without any further modifications.
Legal Information
TargeTron is a trademark of InGex, LLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
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Megan R Kiedrowski et al.
PloS one, 6(11), e26714-e26714 (2011-11-19)
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging contributor to biofilm-related infections. We recently reported that strains lacking sigma factor B (sigB) in the USA300 lineage of CA-MRSA are unable to develop a biofilm. Interestingly, when spent media from a
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