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T8823

Sigma-Aldrich

Monoclonal Anti-T7 tag antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

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Synonym(s):
T7 Tag antibody
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T7 Tag

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

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General description

The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

Antibody is suitable for ELISA and immunoblotting.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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