MilliporeSigma
All Photos(1)

Documents

TRACRRNAMOD

Sigma-Aldrich

SygRNA Cas9 Synthetic Modified tracrRNA

Sign Into View Organizational & Contract Pricing

Select a Size

Change View

Select a Size

Change View
NACRES:
NA.51

packaging

pkg of 1 vial

shipped in

ambient

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
TRACRRNA05NCAS9PROTAB356480
Cas9 Protein from Streptococcus pyogenes, recombinant, expressed in E. coli, 1X NLS

Sigma-Aldrich

CAS9PROT

Cas9 Protein

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

packaging

pkg of 1 vial

packaging

pkg of 5 nmol × Tube (HPLC)

packaging

pkg of 1 kit (3 components)

packaging

antibody small pack of 25 μL

General description

The SygRNA modified synthetic RNA system recruits WT SpCas9, eSpCas9, dCas9, or Cas9D10A to specific genomic targets. 2′ O-methyl and phosphothioate modifications have been incorporated to enhance the stability and performance of the synthetic RNA. The modified tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides SpCas9 and SpCas9 variant proteins to crRNA-specified sites in the genome. SygRNA modified synthetic tracrRNA and crRNAs are optimized for use with a variety of Cas9 expressing cell lines, Cas9 proteins, or Cas9 mRNAs and are compatible with a delivery methods such as microinjection, lipofection, and electroporation.

Application

Functional Genomics/Target Validation/Transgenics

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
  • Generation of transgenic animals via embryo microinjection or electroporation

Features and Benefits

HPLC-purified SygRNA modified tracrRNA oligo is optimized for use with a target-specific crRNA or modified crRNA and a source of SpCas9 protein for genome editing applications.

Linkage

The SygRNA Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein to form active RNP complexes.

Visit SigmaAldrich.com/CRISPRessentials to to review available Cas9 sources.

Order crRNAs at SigmaAldrich.com/CRISPR

Preparation Note

Resuspend SygRNA modified tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

Legal Information

SygRNA is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Protocols

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service