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U5378

Sigma-Aldrich

Urea

powder, BioReagent, for molecular biology, suitable for cell culture

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

product line

BioReagent

Assay

≥98%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

132-135 °C (lit.)

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤1 ppm
Fe: ≤5 ppm
Pb: ≤2 ppm

absorption

≤0.06 at 260 at 6 M
≤0.06 at 280 at 6 M

foreign activity

DNase, NICKase, RNase and protease, none detected

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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This Item
U6504U0631U4884
Urea powder, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

U5378

Urea

Essential+ Grade
Urea suitable for electrophoresis

Sigma-Aldrich

U6504

Urea

-
Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Sigma-Aldrich

U0631

Urea

Premium Grade
Urea meets USP testing specifications

Sigma-Aldrich

U4884

Urea

-
assay

≥98%

assay

≥98%

assay

98.5-101.5%

assay

98.0-102.0%

form

powder

form

powder or crystals

form

powder, crystals or pellets

form

solid

technique(s)

cell culture | mammalian: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

solubility

H2O: 100 mg/mL

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

H2O: soluble 480 g/L at 20 °C

Application

  • Urea has been used in protein resuspension buffer and to denature proteins.
  • It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
  • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Carlson SM and White FM
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Construction and transfection of a ribozyme targeting human caspase-3.
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