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Z374903

Sigma-Aldrich

PCR multiwell plates

96 well plate for PCR

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Synonym(s):
96 mulitwell PCR plate, 96 well PCR microplate, 96 well PCR plate, 96 well microtiter plate
NACRES:
NB.22

material

colorless polypropylene
colorless wells

sterility

non-sterile

feature

plate format: 96 well standard

packaging

case of 25 ea

manufacturer/tradename

Sorenson 2633

technique(s)

PCR: suitable

size

96 well

well maximum volume

300 μm

working volume

200 μL

suitability

suitable for (PCR, RT-PCR or DNA purification applications)

application(s)

agriculture

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This Item
CLS6511Z374911CLS6551
PCR multiwell plates 96 well plate for PCR

Sigma-Aldrich

Z374903

PCR multiwell plates

PCR multiwell plates size 384 wells, polypropylene, skirt, non-sterile

Z374911

PCR multiwell plates

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

feature

plate format: 96 well standard

feature

lid: no, plate format: 96 well Model M

feature

skirt

feature

lid: no, plate format: 96 well standard

packaging

case of 25 ea

packaging

pack of 1, case of 25

packaging

case of 50 ea

packaging

case of 25, pack of 25

manufacturer/tradename

Sorenson 2633

manufacturer/tradename

Corning 6511

manufacturer/tradename

Sorenson 39620

manufacturer/tradename

Corning 6551

size

96 well

size

96 wells

size

384 wells

size

96 wells

General description

A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Features and Benefits

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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Protocols

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.

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