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ZRB1071

Sigma-Aldrich

Anti-Ubiquilin 4 Antibody, clone 5K6 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
A1U, A1Up, Ataxin-1 interacting ubiquitin-like protein, Ataxin-1 ubiquitin-like-interacting protein, CIP75, Connexin43-interacting protein of 75 kDa

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5K6, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 63.85 kDa
observed mol wt ~64 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... UBQLN1(29979)

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recombinant

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2F5, recombinant monoclonal

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 5K6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Ubiquitin 4. It targets an epitope within 25 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human Ubiquitin 4.

Application

Anti-Ubiquilin 4, clone 5K6 ZooMAb, Cat. No. ZRB1071, is a recombinant Rabbit monoclonal antibody that specifically targets Ubiquilin 4 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquilin 4 in Raji and HepG2 cell nuclear extract.

Affinity Binding Assay: A representative lot of this antibody bound Ubiquilin 4 peptide with a KD of 4.0 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquilin 4 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Ubiquilin-4 (UniProt: Q9NRR5; also known as Ataxin-1 interacting ubiquitin-like protein, A1Up, Ataxin-1 ubiquitin-like-interacting protein, A1U, Connexin43-interacting protein of 75 kDa, CIP75) is encoded by the UBQLN4 (also known as C1orf6, CIP75, UBIN) gene (Gene ID: 56893) in human. Ubiquilin-4, a member of the UbL-UBA protein family, serves as an adaptor in the degradation of specific substrates via the proteasomal pathway. It is highly expressed in pancreas, kidney, skeletal muscle, heart, and throughout the brain. Its levels are reported to be upregulated in aggressive tumors. It serves as a regulator of protein degradation and mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. It acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Ubiquilin-4 colocalizes with the proteasome, both in nucleus and cytoplasm and is exported from the nucleus following interaction with DESI1/POST. In response to DNA damage and phosphorylation at serine 318 by ATM, it localizes to the nucleus and is recruited to sites of DNA damage where it represses homologous recombination repair. It acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation then prevents homologous recombination repair and redirects double-strand break repair toward non-homologous end joining. Mutations in UBQLN4 gene are shown to cause Amyotrophic lateral sclerosis (ALS). Defects in UBQLN4 gene also cause intellectual impairment, growth retardation, microcephaly, and facial dysmorphism. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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