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ZRB1535

Sigma-Aldrich

Anti-SLC10A1 Antibody, clone 1B12 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Sodium/bile acid cotransporter;Cell growth-inhibiting gene 29 protein;Na(+)/bile acid cotransporter;Na(+)/taurocholate transport protein;Sodium/taurocholate cotransporting polypeptide;Solute carrier family 10 member 1

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1B12, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 38.12 kDa
observed mol wt ~38 kDa

purified by

using Protein A

species reactivity

human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... SLC10A1(6554)

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Gene Information

human ... SLC10A1(6554)

Gene Information

human ... SLC12A3(6559)

Gene Information

human ... SLC16A1(6566)

Gene Information

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

1B12, recombinant monoclonal

clone

3I12, recombinant monoclonal

clone

2B21, recombinant monoclonal

clone

2E11, recombinant monoclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

rat, human

species reactivity

human, monkey

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Sodium/bile acid cotransporter (SLC10A1). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Sodium/bile acid cotransporter (SLC10A1).

Application

Quality Control Testing

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC10A1 in Human liver tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant Human SLC10A.

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected recombinant Human SLC10A1 protein.

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected SLC10A1 in A549 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC10A1 in Human liver tissue lysate.

Target description

Sodium/bile acid cotransporter (UniProt: Q14973; also known as Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1) is encoded by the SLC10A1 (also known as NTCP, GIG29) gene (Gene ID: 6554) in human. SLC10A1 is a multi-pass membrane glycoprotein with eight transmembrane domains. It is expressed exclusively on the basolateral membrane of hepatocytes and serves as a major high-affinity Na+-dependent transporter of conjugated bile salts from plasma into hepatocytes. It plays a key role in the enterohepatic circulation of bile salts necessary for the solubilization and absorption of dietary fat and fat-soluble vitamins. About 75% of the conjugated bile acids and about 50% of unconjugated bile acids are imported via this transporter. The inward Na+ gradient necessary for this process is maintained by a Na+/K+ ATP pump. This process is shown to be extremely efficient, with 75% to 90% first-pass clearance of bile acids from the sinusoidal blood. SLC10A1 exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. Mutations in SLC10A1 gene are reported to cause familial hypercholanemia 2 (FHCA2) that is characterized by persistently higher plasma levels of conjugated bile salts that is apparent from infancy associated with fat malabsorption and impaired absorption of fat-soluble vitamins. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vaz, FM., et al. (2014). Hepatology. 61(1); 260-267).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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