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ZRB1611

Sigma-Aldrich

Anti-STX17 Antibody, clone 1B3 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Syntaxin 17

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1B3, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 33.4 kDa
observed mol wt ~38 kDa

purified by

using Protein A

species reactivity

mouse, human

species reactivity (predicted by homology)

monkey, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal cytoplasmic domain

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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recombinant

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recombinant

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expressed in HEK 293 cells

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product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Syntaxin 17 (STX17). It targets an epitope within 20 amino acids from the N-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal, cytoplasmic domain of human Syntaxin17 (STX17).

Application

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STX17 in A431 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STX17 in lysates from HepG2 cells, mouse testis tissue, and C2C12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected STX17 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STX17 in A431 and HeLa cells..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Syntaxin-17 (UniProt: P56962; also known as STX17) is encoded by the STX17 gene (Gene ID: 55014) in human. STX17 is a multi-pass membrane protein with two cytoplasmic domains (aa 2-228 and 276-302), two helical domains (aa 229-249 and 255-275), and one lumenal domain (aa 250-254). STX17 is as an autophagosomal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that has a hairpin-type structure mediated by two transmembrane domains, each containing glycine zipper motifs. This structure contributes to its specific localization to completed autophagosomes. Mutations in the glycine residues on the glycine zipper-like motifs is shown to impair its localization to autophagosomes. Although STX17 is shown to localize to the endoplasmic reticulum and mitochondria under nutrient-rich conditions, it translocates to autophagosomes under condition of starvation. It is reported to interact with SNAP29 that is recruited to autophagosomes upon starvation, and VAMP8, the lysosomal SNARE. Depletion of either SNAP29 or VAMP8 is shown to block autophagic flux. STX17 also plays a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment and regulates transport between them. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Viret, C., and Faure, M. (2019). Trends Cell Biol. 29(11); 1-3; Itakura, E., et al. (2012). Cell. 151(6); 1256-1269).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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