MilliporeSigma
All Photos(13)

Documents

ZRB1762

Sigma-Aldrich

Anti-NLRP3 Antibody, clone 4B4 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4B4, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~118.17 kDa
observed mol wt ~95 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ZRB1197ZRB1161ZRB1090
clone

4B4, monoclonal, recombinant monoclonal

clone

4C4, recombinant monoclonal

clone

1D4, monoclonal, recombinant monoclonal

clone

4B5, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

human

species reactivity

human

species reactivity

mouse, human

species reactivity

human, rat

mol wt

calculated mol wt ~118.17 kDa, observed mol wt ~95 kDa

mol wt

calculated mol wt 13.6 kDa, observed mol wt ~15 kDa

mol wt

calculated mol wt ~80.93 kDa, observed mol wt ~110 kDa

mol wt

calculated mol wt ~66.90 kDa, observed mol wt ~60 kDa

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4B4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human NLRP3 protein. It targets an epitope within 15 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal domain of human NLRP3.

Application

Quality Control Testing

Evaluated by Western Blotting in Human Spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NLRP3 in Human Spleen tissue lysate.Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound NLRP3 peptides with a KD of 1.9 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NLRP3 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NLRP3 in THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NLRP3, clone 4B4 ZooMAb®, Cat. No. ZRB1762, is a recombinant Rabbit monoclonal antibody that specifically targets NLRP3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

NACHT, LRR and PYD domains-containing protein 3 (UniProt: Q96P20; also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3) is encoded by the NLRP3 (also known as C1orf7, CIAS1, NALP3, PYPAF1) gene (Gene ID: 114548) in human. NLRP3 serves as the sensor component of the NLRP3 inflammasome and plays a crucial role in innate immunity and inflammation. It is predominantly expressed in macrophages and its expression is also observed in dendritic cells, B- and T-cells, and in lung epithelial cells. NLRP3 can be induced by activators of Toll-like receptors, bacterial lipopolysaccharides, and by TNF. In response to pathogens and other damage-associated signals, it initiates the formation of the inflammasome polymeric complex that is composed of NLRP3, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome is shown to promote its activation and CASP1-catalyzed IL-1B and IL-18 maturation and secretion in the extracellular milieu. Under resting conditions, NLRP3 is autoinhibited and it is activated in response to several signals, including extracellular ATP, reactive oxygen species, K+ efflux, crystals of monosodium urate or cholesterol, and amyloid-beta fibers. Independently of inflammasome activation, it is reported to regulate the differentiation of T helper 2 (Th2) cells and is shown to play a role in Th2 cell-dependent asthma and tumor growth. NLRP3 undergoes polyubiquitination at lysine 48 and 63. However, this ubiquitination does not lead to its degradation, but inhibits inflammasome activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service