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ZRB1779

Sigma-Aldrich

Anti-BSN Antibody, clone 2I2 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2I2, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~416.47 kDa
observed mol wt ~240 kDa

species reactivity

human, rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2I2 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein Bassoon (BSN). It targets an epitope within the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal half of human Protein Bassoon (BSN).

Application

Quality Control Testing

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BSN in U251 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BSN in PC12 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound BSN peptide with a KD of 1.6 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BSN in human cerebral cortex and human retina tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BSN in E18 Rat hippocampal neurons.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-BSN, clone 2I2 ZooMAb®, Cat. No. ZRB1779, is a rabbit recombinant monoclonal antibody that specifically targets Protein Bassoon (BSN) and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Target description

Protein bassoon (UniProt: Q9UPA5; also known as Zinc finger protein 231, BSN) is encoded by the BSN (also known as KIAA0434, ZNF231) gene (Gene ID: 8927) in human. BSN is a multi-domain peripheral membrane protein that is exclusively expressed in brain. It serves as a scaffold protein of the presynaptic cytomatrix at the active zone, the region in the synapse where neurotransmitter is released. At the presynaptic active zone, it regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. It promotes vesicular replenishment and, consequently, a large standing pool of readily releasable synaptic vesicles at the endbulb synapse. BSN are transported on Golgi-derived membranous organelles, called Piccolo-Bassoon transport vesicles (PTVs), from the neuronal soma to distal axonal locations, where they participate in assembling new synapses. It is known to mediate synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import. Mutant mice deficient in functional Bassoon are reported to suffer from epileptic seizures and have dysfunctional hippocampal synapses. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dresbach, T., et al. (2003). Mol. Cell. Neurosci. 23(2); 279-291; Fejtova, A., et al. (2009). J. Cell Biol. 185(2); 341-355; Schultz AM., et al. (2014). EMBO J. 33(4); 512-527).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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