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ZRB1799

Sigma-Aldrich

Anti-Transglutaminase 2 Antibody, clone 1G20 ZooMAb® Rabbit

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G20, recombinant monoclonal

description

1G20 Clone

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 77.34 kDa
observed mol wt ~77 kDa

purified by

using Protein A

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

porcine, bovine, feline, monkey, canine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TGM2(7052)

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recombinant

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ZooMAb® learn more

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ZooMAb® learn more

product line

ZooMAb® learn more

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Transglutaminase 2 (TGM2). It targets an epitope within 21 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Transglutaminase 2.

Application

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Transglutaminase 2 in K562 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Transglutaminase 2 in lysates from C2C12 and L6 cells and Human Placenta tissue.

Affinity Binding Assay: A representative lot of this antibody bound Transglutaminase 2 peptide with a KD of 2.6 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Transglutaminase 2 in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Protein-glutamine gamma-glutamyltransferase 2 (UniProt: P21980; also known as EC: 2.3.2.13, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TG2) is encoded by D13the TGM2 gene (Gene ID: 7052) in human. TG2 is a multi-domain, multi-functional enzyme that is shown to post-translationally modify proteins by catalyzing the formation of intermolecular isopeptide bonds between glutamine and lysine side-chains. It catalyzes the calcium-dependent covalent modification of protein-bound glutamine side chains through transamidation or deamidation reactions. It is mainly localized in the cytoplasm and may be found in the nucleus at much lower levels. Recycling endosomes is reported to be essential for externalization of TG2, which does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. Inactivation of recycling endosomes or blocking endosome fusion with the plasma membrane is shown to block TG2 secretion. TG2 also plays a key role during apoptosis, both by promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm and by mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. It also participates in the pathogenesis of neurodegenerative diseases and certain cancers. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. D12 (Ref.: Zemskov, EA., et al (2011). PLoS ONE 6(4): e19414; Lesort, M., et al. (1998). J. Biol. Chem. 273(20); 11991-11994; Melino, G., et al. ( 1994). Mol. Cell. Biol. 14(10); 6584-6596).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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